Persistencia de enfermedad metastásica ganglionar de cáncer colorrectal con resección en bloque y colocación de injerto aortoilíaco / Persistence of lymph node metastatic disease from colorectal cancer with bloc resection and biaortoiliac graft placement

Introducción: el pronóstico del cáncer de colon está relacionado con el grado de penetración del tumor a través de la pared intestinal y con la infiltración ganglionar en el momento de la cirugía. Se reporta poca experiencia en cuanto al tratamiento quirúrgico de persistencia o recidivas locorregionales a ganglios paraaórticos con afectación de la aorta abdominal. Caso clínico: mujer que presenta persistencia locorregional del cáncer colorrectal con afectación aortoilíaca. Se plantea una estrategia de tratamiento quirúrgico interdisciplinario con intención curativa, con la colocación de un injerto vascular bifurcado y conseguir la resección R0 con la supervivencia libre de enfermedad. Discusión: los pacientes con persistencia de la enfermedad metastásica requieren un abordaje quirúrgico abierto con el objetivo de lograr una resección R0. En el caso de presentarse infiltración local de estructuras a órganos adyacentes, se recomienda la resección radical en bloque. Estos procedimientos más complejos se asocian con una mayor morbilidad. Sin embargo, los beneficios asociados con la eliminación de la metástasis a ganglios paraaórticos deben considerarse frente al riesgo de morbilidad quirúrgica. (AU)

Introduction: the prognosis of colon cancer is related to the degree of tumor penetration through the intestinal wall and lymph node infiltration at the time of surgery. Little experience is reported regarding the surgical treatment of persistence or locoregional recurrences to para-aortic nodes with involvement of the abdominal aorta. Case report: woman presenting with locoregional persistence of colorectal cancer with aortoiliac involvement. An interdisciplinary surgical treatment strategy with curative intent is proposed, with the placement of a vascular graft bifurcated and achieving R0 resection with disease-free survival. Discussion: patients with persistent metastatic disease require an open surgical approach, with the goal of achieving an R0 resection. In the case of local infiltration of structures to adjacent organs, radical en bloc resection is recommended. These more complex procedures are associated with greater morbidity. However, the benefits associated with removal of para-aortic lymph node metastasis must be weighed against the risk of surgical morbidity. (AU)

Character variation in separate body regions of Gerreidae (Osteichthyes: Teleostei) fishes inferred from geometric morphometrics.

Body morphology is a valuable feature for distinguishing teleostean fishes. However, the utility of character variation in separate body regions has yet to be tested. The taxonomy of the Gerreidae family is controversial due to character overlapping among its fish species. This work aims to analyze and compare the body shape variation in three regions, cephalic, trunk, and caudal peduncle, using landmark data and geometric morphometric methods in 17 species and five genera of the family Gerreidae. The pattern of shape variation for the cephalic region consisted of well-defined character states exclusive of each species analyzed. Shape variation in the trunk and caudal peduncle regions does not distinguish all species in this study. This study showed that the dorsal cephalic profile is highly variable among the species, therefore, shape variation in this region is useful for distinguishing Gerreidae species. In contrast, some species within the same genus share similar shape states in the trunk and caudal peduncle regions, with the most shape variation in the dorsal profile and anal fin for the trunk and in the middle of the caudal peduncle.

Derrame pleural unilateral tras migración espontánea de port a cath al hemitóraxizquierdo / Unilateral pleural effusion after spontaneous migration of port a cath to the left hemithorax

Introducción: la migración espontánea del catéter venoso central es una complicación rara de causa desconocidaque ocurre entre el 0,9 y el 2 % de los pacientes. Su etiopatogenia no es muy clara, pero se asocia a eventos dearqueo y vómito intenso que pueden provocar una inversión temporal del fl ujo sanguíneo torácico.Caso clínico: mujer que cursa con diagnóstico de cáncer de mama. Se coloca catéter (port a cath) para quimioterapia. Siete días después ingresa en urgencias por dolor en el hemitórax izquierdo. Se realiza tomografíacomputarizada del tórax y se encuentra una imagen sugestiva de migración de la punta del catéter, con manejopor toracoscopia. Discusión: un acceso vascular central es importante para los pacientes que necesitan recibir tratamiento médicooncológico. es un procedimiento frecuente que en la actualidad se relaciona con una mayor comodidad parael paciente, ya que, una vez que se inserta quirúrgicamente, puede usarse varias veces convenientemente. Sinembargo, no está exento de complicaciones. estas pueden dividirse en tempranas y tardías. Las primeras puedenincluir punciones arteriales, malposiciones, neumotórax o hemotórax. La infección y la trombosis del catéter sonlas complicaciones tardías más frecuentes. en cambio, la migración espontánea de la punta del catéter se reportacomo una complicación infrecuente.(AU)

Introduction: spontaneous central venous catheter migration is a rare complication of unknown cause that occurs in about 0.9 to 2 % of patients. its etiopathogenesis is not very clear, however, it is associated with retching eventsand intense vomiting that can cause a temporary inversion of thoracic blood flow. Case report: women with a diagnosis of breast cancer, a catheter (port a cath) was placed for chemotherapy,7 days later she was admitted to the emergency department due to pain in the left hemithorax, a chest com-puted tomography was performed, finding an image suggestive of migration of the breast. catheter tip, withmanagement by thoracoscopy. Discussion: a central vascular access is important for patients who need to receive medical oncological treatment,being a common procedure that is currently related to greater comfort for the patient, since, once it is surgicallyinserted, it can be conveniently used several times. However, it is not without complications. these can be dividedinto early and late. the former may include arterial punctures, malpositions, pneumothorax or hemothorax, andinfection and catheter thrombosis are the most common late complications. on the other hand, spontaneousmigration of the catheter tip is reported as a rare complication.(AU)

Legionella and mitochondria, an intriguing relationship.

Legionella pneumophila is the causative agent of Legionnaires' disease, a severe pneumonia. L. pneumophila injects via a type-IV-secretion-system (T4SS) more than 300 bacterial proteins into macrophages, its main host cell in humans. Certain of these bacterial effectors target organelles in the infected cell and hijack multiple processes to facilitate all steps of the intracellular life cycle of this pathogen. In this review, we discuss the interplay between L. pneumophila, an intracellular bacterium fully armed with virulence tools, and mitochondria, the extraordinary eukaryotic organelles playing prominent roles in cellular bioenergetics, cell-autonomous immunity and cell death. We present and discuss key findings concerning the multiple interactions of L. pneumophila with mitochondria during infection and the mechanisms employed by T4SS effectors that target mitochondrial functions to subvert infected cells.

High-content assay to measure mitochondrial function and bacterial vacuole size in infected human primary macrophages.

Regulation of bioenergetics and cell death are pivotal mitochondrial functions determining the responses of macrophages to infection. Here, we provide a protocol to investigate mitochondrial functions during infection of macrophages by intracellular bacteria. We describe steps for quantifying mitochondrial polarization, cell death, and bacterial infection in infected, living, human primary macrophages at the single-cell level. We also detail the use of the pathogen Legionella pneumophila as model. This protocol can be adapted to investigate mitochondrial functions in other settings. For complete details on the use and execution of this protocol, please refer to Escoll et al. (2021).1.

2-deoxyglucose transiently inhibits yeast AMPK signaling and triggers glucose transporter endocytosis, potentiating the drug toxicity.

2-deoxyglucose is a glucose analog that impacts many aspects of cellular physiology. After its uptake and its phosphorylation into 2-deoxyglucose-6-phosphate (2DG6P), it interferes with several metabolic pathways including glycolysis and protein N-glycosylation. Despite this systemic effect, resistance can arise through strategies that are only partially understood. In yeast, 2DG resistance is often associated with mutations causing increased activity of the yeast 5'-AMP activated protein kinase (AMPK), Snf1. Here we focus on the contribution of a Snf1 substrate in 2DG resistance, namely the alpha-arrestin Rod1 involved in nutrient transporter endocytosis. We report that 2DG triggers the endocytosis of many plasma membrane proteins, mostly in a Rod1-dependent manner. Rod1 participates in 2DG-induced endocytosis because 2DG, following its phosphorylation by hexokinase Hxk2, triggers changes in Rod1 post-translational modifications and promotes its function in endocytosis. Mechanistically, this is explained by a transient, 2DG-induced inactivation of Snf1/AMPK by protein phosphatase 1 (PP1). We show that 2DG-induced endocytosis is detrimental to cells, and the lack of Rod1 counteracts this process by stabilizing glucose transporters at the plasma membrane. This facilitates glucose uptake, which may help override the metabolic blockade caused by 2DG, and 2DG export-thus terminating the process of 2DG detoxification. Altogether, these results shed a new light on the regulation of AMPK signaling in yeast and highlight a remarkable strategy to bypass 2DG toxicity involving glucose transporter regulation.

Morphological abnormalities in seven American round ray specimens: A review of America's batomorph anomalies.

Although morphological abnormalities in several rays and skate species around the American continents have frequently mentioned, their numbers are unknown. The present work record morphological abnormalities in four Urotrygonidae species. Two anophthalmic specimens were detected (Urotrygon microphthalmum and Urobatis halleri). Two individuals lacked caudal fins (Urobatis maculatus and Urotrygon chilensis). Two round rays showed incomplete fusion of the pectoral fin to the head (U. microphthalmum and U. chilensis). Vertebral compression and fusion were found in a 6-year-old female Urotrygon rogersi. In addition, 118 abnormal batomorph specimens were gathered from the available bibliography, spanning the last six decades (1959-2021). Amblyraja doellojuradoi was the species with the highest number of abnormalities (18). The most common anomaly was an incomplete fusion of the pectoral fin with the head. Since 2010, at least 30 anomalous batomorphs have been recorded every 5 years. Sixty-nine abnormal specimens occurred in the Northern Hemisphere (1.00-60.00 N). The Cortezian (Pacific) and Southeastern Brazil (Atlantic) marine ecoregions stood out with the highest number of these specimens. Mexico recorded 58 anomalous specimens, followed by Brazil (n = 36). Biological, abiotic and anthropogenic factors are probably the leading causes. However, additional studies are necessary to elucidate these speculations.

New microsatellite loci for estimating genetic diversity and structure in Octopus hubbsorum from Nayarit, México.

BACKGROUND: Octopus hubbsorum Berry, 1953 is the most important species for commercial fishing in the Mexican Pacific. However, there is a lack of information regarding population structure that could have important management implications. We tested 44 microsatellite loci in O. hubbsorum by cross-amplification from O. bimaculatus. METHODS AND RESULTS: Genetic diversity and structure was tested over 30 octopus sampled from Santa Cruz de Miramar (Nayarit, México). A total of 11 loci were successfully amplified. All loci were polymorphic with the number of effective alleles ranging from 2.13 to 23.14, while three loci significantly deviated from Hardy-Weinberg equilibrium. No significant LD was observed between pairs of loci (P ≥ 0.05). The application of the new markers in a O. hubbsorum population from Santa Cruz de Miramar Nayarit, México, did not showed Wahlund or isolate breaking effects due to the mixing of distinct populations. CONCLUSIONS: The loci were useful to estimate levels of pairwise relatedness and to discard the presence of recent demographic bottlenecks in the population. We consider that eight microsatellites are adequate from the 11 amplified loci.

Revision of the diagnostic characters of two morphologically similar snook species, Centropomus viridis and C. nigrescens (Carangiformes: Centropomidae).

Historically, the taxonomic identification of the two snook species, Centropomus viridis and C. nigrescens, has been challenging due to their morphological similarity and the inconsistency of the characters used for diagnosis. Therefore, this study aimed to evaluate the usefulness of the morphologic, meristic, and morphometric characters currently being used to identify C. viridis and C. nigrescens, based on molecular data. The results showed that the gas-bladder shape (i.e., C. viridis with diverticula and C. nigrescens without diverticula) was the only morphological character univocally related to genetic identification. Likewise, geometric morphometrics separated two groups; each corresponds to only one of two genetically (and gas bladder shape) identified species. Of all the meristic characters examined, only the second dorsal fin ray count (nine for C. viridis and ten for C. nigrescens) was related to the gas bladder shape and genetic identity; therefore, it is the only external character with a diagnostic utility to separate each species.

Preservación de la fertilidad: opinión de grupo de expertos en México / Fertility preservation: an expert taskforce opinion

La preservación de la fertilidad es la aplicación de estrategias médicas y de laboratorio para preservar la descendencia genética parental en adultos o niños en riesgo de esterilidad. El cáncer es la principal indicación de preservación de fertilidad en pacientes en edad reproductiva. En las últimas décadas ha incrementado la incidencia de cáncer en adolescentes. Los tratamientos oncológicos también han mejorado significativamente, por lo que hoy es posible la curación en un amplio porcentaje de pacientes. La mayoría de los niños y adolescentes con cáncer se convierten en sobrevivientes a largo plazo, lo que aumenta el interés en los efectos del tratamiento del cáncer sobre la fertilidad. Las condiciones sociales, económicas y culturales también son determinantes para decidir el momento que una pareja busque promover su fertilidad. Además, otras patologías o incluso fármacos para prevención del rechazo de órganos trasplantados pueden afectar la fertilidad y, por tanto, tales pacientes son susceptibles de orientación sobre preservación de la fertilidad. El éxito en los programas de reproducción asistida y en los tratamientos oncológicos brindan alternativas para preservar la fertilidad. En esta primera Opinión de Grupo de Expertos Mexicanos en Preservación de la Fertilidad hemos evaluado pacientes oncológicas que son candidatas a preservación de fertilidad: jóvenes con riesgo de compromiso de su fertilidad por el tratamiento oncológico, pero con reserva ovárica suficiente y pronóstico vital aceptable. También se consideraron casos especiales como la preservación social, en casos de conceptualización sexual diferente, así como los aspectos legales y éticos básicos

Fertility preservation is the application of medical and laboratory strategies to preserve parental genetic offspring in adults or children at risk of sterility. Cancer is the main indication of fertility preservation in patients of reproductive age. In recent decades, the incidence of cancer in adolescents has increased. Cancer treatments have also improved significantly, making cure possible today in a large percentage of patients. Most children and adolescents with cancer become long-term survivors, increasing interest in the effects of cancer treatment on fertility. Social, economic and cultural conditions are also decisive in deciding when a couple seeks to promote their fertility. Furthermore, other pathologies or even drugs for the prevention of rejection of transplanted organs can affect fertility and, therefore, such patients are susceptible to guidance on fertility preservation. Success in assisted reproduction programs and cancer treatments provide alternatives to preserve fertility. In this first Opinion of the Group of Mexican Experts on Fertility Preservation, we have evaluated oncological patients who are candidates for fertility preservation: young people at risk of compromising their fertility due to oncological treatment, but with sufficient ovarian reserve and acceptable vital prognosis. Special cases such as social preservation were also considered, in cases of different sexual conceptualization, as well as the basic legal and ethical aspects

Taxonomic assessment of species of the genus Octopus from the northeastern Pacific via morphological, molecular and morphometric analyses.

Species of the genus Octopus from the northeastern Pacific are ecologically and economically important; however, their taxonomy is confusing and has not been comprehensively assessed. In this study, we performed a taxonomic evaluation of these species considering the morphological characteristics of the original descriptions, a molecular analysis of partial COI-gene sequences, and a traditional morphometry analysis of nine body measurements. Several interesting findings were obtained with our results: for instance, we updated the diagnoses of some species by including characters such as the number of lamellae per demibranch and the presence of chromatophores in the visceral sac; we deposited partial COI-gene sequences of species that had not been incorporated into the GenBank repository; and according to the morphometric analysis, we confirmed that the lengths of arms I-IV are relevant to discriminate the species under study. The taxa evaluated were morphologically, molecularly and morphometrically well-delimited; however, features such as funnel organ shape and arm length proportions in regard to dorsal mantle length are either not included in the diagnosis of the genus Octopus or overlap with other genera. Hence, this information, combined with the results obtained from the molecular analysis, supports the generic re-assignation of two of the species evaluated.

Genetic homogeneity of the Pacific thread herring (Opisthonema libertate) (Günther, 1867) in the Eastern Pacific, inferred from mtDNA sequences.

In the present study, the population genetic structure of the Pacific thread herring (Opisthonema libertate) was analyzed through mitochondrial DNA (mtDNA) control region sequences. Organisms were collected from June 2015 to July 2015 from four commercial landing sites (Bahia Magdalena, Guaymas and Mazatlan, Mexico, and Puntarenas, Costa Rica) and one artisanal sampling site (Puerto La Libertad, El Salvador). A total of 125 sequences were analyzed. High levels of haplotype (h = 0.990) and nucleotide (π = 0.030) diversity were found. Pairwise Φst comparisons indicated differences attributed mainly to the organisms from El Salvador. However, Bayesian inferences did not support the existence of different populations. The haplotype distribution between locations did not show a clear phylogeographic pattern. Mismatched distribution showed a unimodal pattern for the five sampled areas, indicative of sudden demographic expansion. These results were supported by Bayesian skyline plot. Our results do not support the hypothesis that the Pacific thread herring presents a population genetic structure. Future genetic comparisons should include a larger number of samples as well as more polymorphic molecular markers to further support our results.

Genetic and cellular sensitivity of Caenorhabditis elegans to the chemotherapeutic agent cisplatin.

Cisplatin and derivatives are commonly used as chemotherapeutic agents. Although the cytotoxic action of cisplatin on cancer cells is very efficient, clinical oncologists need to deal with two major difficulties, namely the onset of resistance to the drug and the cytotoxic effect in patients. Here, we used Caenorhabditis elegans to investigate factors influencing the response to cisplatin in multicellular organisms. In this hermaphroditic model organism, we observed that sperm failure is a major cause of cisplatin-induced infertility. RNA sequencing data indicate that cisplatin triggers a systemic stress response, in which DAF-16/FOXO and SKN-1/NRF2, two conserved transcription factors, are key regulators. We determined that inhibition of the DNA damage-induced apoptotic pathway does not confer cisplatin protection to the animal. However, mutants for the pro-apoptotic BH3-only gene ced-13 are sensitive to cisplatin, suggesting a protective role of the intrinsic apoptotic pathway. Finally, we demonstrated that our system can also be used to identify mutations providing resistance to cisplatin and therefore potential biomarkers of innate cisplatin-refractory patients. We show that mutants for the redox regulator trxr-1, ortholog of the mammalian thioredoxin reductase 1 TRXR1, display cisplatin resistance. By CRISPR/Cas9, we determined that such resistance relies on the presence of the single selenocysteine residue in TRXR-1.This article has an associated First Person interview with the first author of the paper.

Orthoxenografts of Testicular Germ Cell Tumors Demonstrate Genomic Changes Associated with Cisplatin Resistance and Identify PDMP as a Resensitizing Agent.

Purpose: To investigate the genetic basis of cisplatin resistance as efficacy of cisplatin-based chemotherapy in the treatment of distinct malignancies is often hampered by intrinsic or acquired drug resistance of tumor cells.Experimental Design: We produced 14 orthoxenograft transplanting human nonseminomatous testicular germ cell tumors (TGCT) in mice, keeping the primary tumor features in terms of genotype, phenotype, and sensitivity to cisplatin. Chromosomal and genetic alterations were evaluated in matched cisplatin-sensitive and their counterpart orthoxenografts that developed resistance to cisplatin in nude mice.Results: Comparative genomic hybridization analyses of four matched orthoxenografts identified recurrent chromosomal rearrangements across cisplatin-resistant tumors in three of them, showing gains at 9q32-q33.1 region. We found a clinical correlation between the presence of 9q32-q33.1 gains in cisplatin-refractory patients and poorer overall survival (OS) in metastatic germ cell tumors. We studied the expression profile of the 60 genes located at that genomic region. POLE3 and AKNA were the only two genes deregulated in resistant tumors harboring the 9q32-q33.1 gain. Moreover, other four genes (GCS, ZNF883, CTR1, and FLJ31713) were deregulated in all five resistant tumors independently of the 9q32-q33.1 amplification. RT-PCRs in tumors and functional analyses in Caenorhabditis elegans (C. elegans) indicate that the influence of 9q32-q33.1 genes in cisplatin resistance can be driven by either up- or downregulation. We focused on glucosylceramide synthase (GCS) to demonstrate that the GCS inhibitor DL-threo-PDMP resensitizes cisplatin-resistant germline-derived orthoxenografts to cisplatin.Conclusions: Orthoxenografts can be used preclinically not only to test the efficiency of drugs but also to identify prognosis markers and gene alterations acting as drivers of the acquired cisplatin resistance. Clin Cancer Res; 24(15); 3755-66. ©2018 AACR.

Life histories predict genetic diversity and population structure within three species of octopus targeted by small-scale fisheries in Northwest Mexico.

The fishery for octopus in Northwest Mexico has increased to over 2,000 tons annually, but to date the specific composition of the catch has been ignored. With at least three main species targeted by artisanal fisheries in the region with distinct life histories, the lack of basic biological information about the distribution, metapopulation size and structure of each species could impede effective fisheries management to avoid overexploitation. We tested if different life histories of three species of octopus could help predict observed patterns of genetic diversity, population dynamics, structure and connectivity and how this information could be relevant to the sustainable management of the fishery. We sequenced two mitochondrial genes and genotyped seven nuclear microsatellite loci to identify the distribution of each species in 20 locations from the Gulf of California and the west coast of the Baja California peninsula. We tested five hypotheses derived from population genetic theory based on differences in the fecundity and dispersal potential for each species. We discovered that Octopus bimaculoides with low fecundity and direct development (without a planktonic phase) had lower average effective population size and genetic diversity, but higher levels of kinship, population structure, and richness of private alleles, than the other two species. These features indicated limited dispersal and high local recruitment. In contrast, O. bimaculatus and O. hubbsorum with higher fecundity and planktonic phase as paralarvae had higher effective population size and genetic diversity, and overall lower kinship and population structure than O. bimaculoides. These observations supported higher levels of gene flow over a larger geographical scale. O. bimaculatus with the longest planktonic paralarval duration and therefore larger dispersal potential had differences in the calculated parameters possibly associated with increased connectivity. We propose O. bimaculoides is more susceptible to over exploitation of small, isolated populations and could have longer recovery times than the other two species. This species may benefit from distinct fishery management within each local population. O. bimaculatus and O. hubbsorum may benefit from fishery management that takes into account metapopulation structure over larger geographic scales and the directionality and magnitude of larval dispersal driven by ocean currents and population connectivity among individuals of each locality. The distribution of each species and variations in their reproductive phenology is also important to consider when establishing marine reserves or seasonal fishing closures.

Overexpression of MMP-3 and uPA with Diminished PAI-1 Related to Metastasis in Ductal Breast Cancer Patients Attending a Public Hospital in Mexico City.

Extracellular matrix metalloproteases and the fibrinolytic system are important protease systems interacting with each other in charge of remodeling and recycling of tissues. Their role in tumor invasion and metastasis is often discussed. In this study several metalloproteases such as MMP-1, MMP-3, MMP-9, and TIMP-1 together with molecules from the fibrinolytic system like uPA, its receptor uPAR, and its inhibitor, PAI-1, were studied by immune-histochemistry to establish a comparison with and without metastasis. From the (118) primary tumors of Mexican patients with ductal breast cancer studied, 56% were grade II and 69% were size T2; the group with metastatic ganglia included 64 samples (54.3%). In patients with metastasis the estimated expression of MMP-3 and uPA (resp., 28% and 45%) was higher than that from no metastatic tumors; it means there is higher expression of both markers in metastatic tumors (p < 0.05). At the same time, metastatic tumors showed statistically significant lower signal of PAI-1 (24%) than tumors without metastasis (p < 0.05). We concluded that overexpression of MMP-3 and uPA, altogether with diminished expression of PAI-1 from metastatic tumors, might be a crucial step towards metastasis in ductal breast cancer. Nevertheless, additional studies in different populations are necessary to establish a pattern.

Intervención cognitivo-conductual para el control de ansiedad ante la biopsia insicional en pacientes con cáncer de mama / Cognitive-behavioral intervention to control anxiety about incisional biopsy in patients with breast cancer

La atención del enfermo oncológico implica la realización de pruebas médico invasivas para la obtención de un diagnóstico preciso. Cuando los pacientes se someten a estos procedimientos experimentan reacciones desadaptativas como miedo, ansiedad y dolor. En el caso del cáncer de mama, la prueba diagnóstica más utilizada es la biopsia incisional, procedimiento que las pacientes ignoran y que condiciona la presencia de incertidumbre, ansiedad y pensamientos anticipados negativos respecto al procedimiento y al posible resultado. La ansiedad y el dolor pueden provocar complicaciones fisiológicas, conductuales y emocionales, motivo por el cual el psicólogo especialista en medicina conductual participa antes, durante y después de la toma de biopsia. El objetivo de este estudio fue evaluar el efecto de un programa cognitivo-conductual sobre la ansiedad en mujeres que se sometieron a toma de biopsia incisional en mama por primera vez. Participaron 10 pacientes del servicio de Oncología del Hospital Juárez de México las cuales fueron atendidas en la consulta externa. La intervención consistió en proporcionar psicoeducación y entrenamiento en relajación pasiva a través de videos, grabaciones e información impresa. Para evaluar ansiedad se utilizaron el inventario IDARE-Estadoy la Escala de Expresión Facial de la Ansiedad (EEF-A), así como tensión arterial y frecuencia cardíaca antes y después de la intervención. Los datos fueron analizados tanto intrasujeto como entre sujetos mediante la prueba de Wilcoxon (p≤0,05) encontrándose reducción de la ansiedad como grupo en lasescalas de ansiedad-estado (puntajes crudos y rangos) y en la EEF-A

Oncological patients are submitted to invasive exams in order to obtain an accurate diagnosis; these procedures may cause maladaptative reactions (fear, anxiety and pain). Particularly in breast cancer, the most common diagnose technique is the incisional biopsy. Most of the patients are unaware about the procedure and for that reason they may focus their thoughts on possible events such as pain, bleeding, the anesthesia, or the later surgical wound care. Anxiety and pain may provoke physiological, behavioral and emotional complications, and because of this reason, the Behavioral Medicine trained psychologist takes an active role before and after the biopsy. The aim of this study was to evaluate the effect of a cognitive-behavioral program to reduce anxiety in women submitted to incisional biopsy for the first time. There were 10 participants from the Hospital Juárez de México, Oncology service; all of them were treated as external patients. The intervention program focused in psycho-education and passive relaxation training using videos, taperecorded instructions and pamphlets. Anxiety measures were performed using the IDAREState inventory, and a visual-analogue scale of anxiety (EEF-A), and the measurement of blood pressure and heart rate). Data were analyzed both intrasubject and intersubject using the Wilcoxon test (p≤00.05). The results show a reduction in anxiety (as in punctuation as in ranges) besides, a reduction in the EEF-A

[Clear cell adenocarcinoma arising from abdominal wall endometriosis]. / Adenocarcinoma de células claras originado de endometriosis en pared abdominal.

BACKGROUND: Clear cell carcinoma originating in the abdominal wall is a rare event. It is generally associated with endometrial tissue implants left behind after a caesarean section or other gynaecological operations. Its pathophysiology is complex and controversial. CLINICAL CASE: The case is presented of a 45 year-old female with history of three caesarean sections, who was seen due to having a tumour mass of 6 months onset in the anterior abdominal wall. Imaging studies confirmed its location, and due to measuring 9 by 7 cm it was suspected to be an urachal tumour. A resection with wide margins was performed. The histopathology report was of a clear cell adenocarcinoma originated in ectopic endometrial tissue, with negative margins. CONCLUSION: This is a very rare case, with few cases reported in the literature. This diagnosis should be included in tumours of the abdominal wall.

The complete mitochondrial genome of Octopus bimaculatus Verrill, 1883 from the Gulf of California.

The complete mitochondrial genome of Octopus bimaculatus is 16 085 bp in length and includes 13 protein-codes genes, 2 ribosomal RNA genes, 22 transfers RNA genes, and a control region. The composition of genome is A (40.9%), T (34.7%), C (16.9%), and G (7.5%). The control region of O. bimaculatus contains a VNTR locus not present in the genomes from other octopus species. A phylogenetic analysis shows a closer relationship between the mitogenomes from O. bimaculatus and O. vulgaris.

Modeling of autosomal-dominant retinitis pigmentosa in Caenorhabditis elegans uncovers a nexus between global impaired functioning of certain splicing factors and cell type-specific apoptosis.

Retinitis pigmentosa (RP) is a rare genetic disease that causes gradual blindness through retinal degeneration. Intriguingly, seven of the 24 genes identified as responsible for the autosomal-dominant form (adRP) are ubiquitous spliceosome components whose impairment causes disease only in the retina. The fact that these proteins are essential in all organisms hampers genetic, genomic, and physiological studies, but we addressed these difficulties by using RNAi in Caenorhabditis elegans. Our study of worm phenotypes produced by RNAi of splicing-related adRP (s-adRP) genes functionally distinguishes between components of U4 and U5 snRNP complexes, because knockdown of U5 proteins produces a stronger phenotype. RNA-seq analyses of worms where s-adRP genes were partially inactivated by RNAi, revealed mild intron retention in developing animals but not in adults, suggesting a positive correlation between intron retention and transcriptional activity. Interestingly, RNAi of s-adRP genes produces an increase in the expression of atl-1 (homolog of human ATR), which is normally activated in response to replicative stress and certain DNA-damaging agents. The up-regulation of atl-1 correlates with the ectopic expression of the pro-apoptotic gene egl-1 and apoptosis in hypodermal cells, which produce the cuticle, but not in other cell types. Our model in C. elegans resembles s-adRP in two aspects: The phenotype caused by global knockdown of s-adRP genes is cell type-specific and associated with high transcriptional activity. Finally, along with a reduced production of mature transcripts, we propose a model in which the retina-specific cell death in s-adRP patients can be induced through genomic instability.